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a) Volcano plot representation of miRNA expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis

Journal: bioRxiv

Article Title: Microglia-derived extracellular vesicles attenuate acute a-synuclein induced astrocyte inflammation

doi: 10.64898/2026.05.11.724371

Figure Lengend Snippet: a) Volcano plot representation of miRNA expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis

Article Snippet: Cells were transfected 24 hours prior to additional treatments. miRNA mimics for mmu-miR-5099 (MED Chem express, Catalog# HY-R03245), mmu-miR-720 (Thermofisher, Catalog # 4427975) which has the same mature sequence as target Novel-miRNA-115 identified though miRNA sequencing, and scrambled miRNA (MCE, Catalog # HY-R04602 Lot#825593) were utilized for the transfections.

Techniques: Expressing, Derivative Assay, Sequencing